Coding

Part:BBa_J56002

Designed by: Matt Eames   Group: iGEM06_UCSF   (2006-10-13)

Tar EA400 has orthogonal interaction with CheW VM108

Tar (aspartate-sensitive chemotaxis receptor) mutated (EA400) to have orthogonal interaction with CheW VM108. Has minimal interaction with wild-type CheW. Mutation was orginally discovered in the serine receptor Tsr (see reference). Orthogonal mutation was mapped onto Tar. Sequence has been codon-optimized to avoid recombination with similar sequences in system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 181
    Illegal PstI site found at 1304
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1456
    Illegal PstI site found at 181
    Illegal PstI site found at 1304
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1663
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 181
    Illegal PstI site found at 1304
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 181
    Illegal PstI site found at 1304
    Illegal NgoMIV site found at 1605
    Illegal AgeI site found at 1282
  • 1000
    COMPATIBLE WITH RFC[1000]


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